Population Dynamics of Gamma Proteobacteria: Critical Analysis during Different Phases of Composting

Document Type

Book Chapter

Publication Title

Analytical Case Studies on Municipal and Biomedical Waste Management Perspectives on Sustainable Development Goals

Abstract

The fourth chapter presents analytical data regarding the population dynamics of ?- Proteobacteria during different phases of composting using flow cytometry. Studies have shown that members of phylum Proteobacteria predominate the process of composting. Composting refers to the process of converting organic wastes into nutrient-rich soil through natural decomposition. Waste valorization is the process of reusing, recycling or composting waste materials and converting them into more useful products including materials, chemicals, fuels or other sources of energy. In the present study, a composting pile was set up with a mixture of vegetable waste, rice straw and cow dung (ratio-5:1:02). Samples were collected from three different phases of composting: initial phase, thermophilic phase and cooling or maturation phase. Total cell count and ?- Proteobacteria population were enumerated by flow cytometry. Results showed the population of ?- Proteobacteria decreased during the thermophilic stage. A strong negative correlation was observed between temperature and percentage of ?- Proteobacteria population, indicating that members of this subgroup are essentially mesophiles. The case study portrays the data through detailed confocal micrographs and cytograms. The chapter focuses on addressing SDG 2 (Zero Hunger), SDG 3 (Good Health and Wellbeing), SDG 7 (Affordable and Clean Energy), SDG 9 (Industry, Innovation, and Infrastructure), SDG 12 (Responsible Consumption and Production), and SDG 15 (Life on Land).

First Page

43

Last Page

54

DOI

10.1201/9781003499695-4

Publication Date

1-1-2024

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