Evaluation of the growth response of spore forming lactic acid Bacillus–Bacillus coagulans in presence of oxide nanoparticles

Article Type

Research Article

Publication Title

Applied Nanoscience (Switzerland)

Abstract

It is important to develop fundamental understanding on the utilization of inorganic nanoparticles (NPs) in health and environment as their use in the agricultural food sector, medical applications, cosmetics, paints are growing rapidly. Agricultural food sector is one of the major fields where these particles of less than 100 nm diameter are applied. This sector uses this revolutionary technology for increased growth of crop; thus, its effect on human body not only need to be studied, but also its effect on human beneficial gut flora that are in symbiosis relationship with human kind needs attention. Here, experiments were conducted on one of the dominant beneficial human gut bacteria (spore-forming lactic acid Bacillus, Bacillus coagulans) in presence of both titanium dioxide (TiO2) anatase nanoparticles (as this nanomaterial used mostly in plants among metal-based nanomaterials) and zinc oxide (ZnO) NPs (as this nanomaterial used mostly in drugs as antimicrobial agent to destroy harmful bacteria, nowadays also used as antiviral agent). ZnO NPs show toxicity at and above 1 mg/L, whereas bacteria show more growth than control in presence of TiO2 anatase NP at 1 mg/L in absence of light. TEM and SEM images and results of enzyme assay revealed that NPs do not aggregate, they act on bacteria, but bacterial cell wall integrity behave differently in presence of different NPs with high level of control on regulation of energy; hence, nanoparticle interaction mechanism with bacteria is studied thoroughly.

First Page

4075

Last Page

4086

DOI

10.1007/s13204-020-01512-w

Publication Date

11-1-2020

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