A phase 2 single center open label randomised control trial for convalescent plasma therapy in patients with severe COVID-19

Authors

Yogiraj Ray, Infectious Disease & Beleghata General Hospital
Shekhar Ranjan Paul, Infectious Disease & Beleghata General Hospital
Purbita Bandopadhyay, Indian Institute of Chemical Biology
Ranit D’Rozario, Indian Institute of Chemical Biology
Jafar Sarif, Indian Institute of Chemical Biology
Deblina Raychaudhuri, Indian Institute of Chemical Biology
Debaleena Bhowmik, Academy of Scientific and Innovative Research (AcSIR)
Abhishake Lahiri, Academy of Scientific and Innovative Research (AcSIR)
Janani Srinivasa Vasudevan, Institute of Genomics and Integrative Biology India
Ranjeet Maurya, Institute of Genomics and Integrative Biology India
Akshay Kanakan, Institute of Genomics and Integrative Biology India
Sachin Sharma, Institute of Genomics and Integrative Biology India
Manish Kumar, Institute of Genomics and Integrative Biology India
Praveen Singh, Institute of Genomics and Integrative Biology India
Rammohan Roy, Infectious Disease & Beleghata General Hospital
Kausik Chaudhury, Infectious Disease & Beleghata General Hospital
Rajsekhar Maiti, Infectious Disease & Beleghata General Hospital
Saugata Bagchi, Infectious Disease & Beleghata General Hospital
Ayan Maiti, Infectious Disease & Beleghata General Hospital
Md Masoom Perwez, Infectious Disease & Beleghata General Hospital
Abhinandan Mondal, Infectious Disease & Beleghata General Hospital
Avinash Tewari, Infectious Disease & Beleghata General Hospital
Samik Mandal, Infectious Disease & Beleghata General Hospital
Arpan Roy, Infectious Disease & Beleghata General Hospital
Moumita Saha, Infectious Disease & Beleghata General Hospital
Durba Biswas, Medical College and Hospital Kolkata
Chikam Maiti, Medical College and Hospital Kolkata
Ritwik Bhaduri, Indian Statistical Institute, Kolkata
Sayantan Chakraborty, Tata Medical Center
Biswanath Sharma Sarkar, Infectious Disease & Beleghata General Hospital
Anima Haldar, Infectious Disease & Beleghata General Hospital
Bibhuti Saha, Calcutta School of Tropical Medicine
Shantanu Sengupta, Academy of Scientific and Innovative Research (AcSIR)

Article Type

Research Article

Publication Title

Nature Communications

Abstract

A single center open label phase 2 randomised control trial (Clinical Trial Registry of India No. CTRI/2020/05/025209) was done to assess clinical and immunological benefits of passive immunization using convalescent plasma therapy. At the Infectious Diseases and Beleghata General Hospital in Kolkata, India, 80 patients hospitalized with severe COVID-19 disease and fulfilling the inclusion criteria (aged more than 18 years, with either mild ARDS having PaO2/FiO2 200–300 or moderate ARDS having PaO2/FiO2 100–200, not on mechanical ventilation) were recruited and randomized into either standard of care (SOC) arm (N = 40) or the convalescent plasma therapy (CPT) arm (N = 40). Primary outcomes were all-cause mortality by day 30 of enrolment and immunological correlates of response to therapy if any, for which plasma abundance of a large panel of cytokines was quantitated before and after intervention to assess the effect of CPT on the systemic hyper-inflammation encountered in these patients. The secondary outcomes were recovery from ARDS and time taken to negative viral RNA PCR as well as to report any adverse reaction to plasma therapy. Transfused convalescent plasma was characterized in terms of its neutralizing antibody content as well as proteome. The trial was completed and it was found that primary outcome of all-cause mortality was not significantly different among severe COVID-19 patients with ARDS randomized to two treatment arms (Mantel-Haenszel Hazard Ratio 0.6731, 95% confidence interval 0.3010-1.505, with a P value of 0.3424 on Mantel-Cox Log-rank test). No adverse effect was reported with CPT. In severe COVID-19 patients with mild or moderate ARDS no significant clinical benefit was registered in this clinical trial with convalescent plasma therapy in terms of prespecified outcomes.

DOI

10.1038/s41467-022-28064-7

Publication Date

12-1-2022

Comments

Open Access, Gold, Green

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