Chemical profiling, cytotoxicity study and assessment of antioxidant potential of hydro-ethanol extract of peels of some selected varieties of potato in various in vitro models and in lipid substrate enriched with omega-3 fatty acids

Article Type

Research Article

Publication Title

European Food Research and Technology


Omega-3 fatty acids have immense health benefits. But developing omega-3 fatty acids fortified food is a challenging task. The aim of this study was to evaluate possible antioxidant potential of hydro ethanol extract of peels of five selected varieties of potatoes (Kufri Chipsona-1, Kufri Chipsona-3, Kufri Chipsona-4, Kufri Chandramukhi and Kufri Jyoti) in various in vitro models and in lipid substrate enriched with omega-3 fatty acids. In vitro antioxidant potential was evaluated by DPPH radical scavenging, β-carotene linoleic acid bleaching and Fe2+ ion chelating methods. Anti-lipid peroxidation potential was evaluated in omega-3 fatty acids-enriched bulk oil and oil-in-water emulsion model systems using peroxide value (PV), p-anisidine value (p-AV) and TOTOX value as indicators of oxidation. Chemical characterization of compounds was performed by LC–ESI–MS/MS analysis and cytotoxic potential was evaluated by brine shrimp lethality assay. Among the peel extracts tested, Kufri Chipsona-3 potato peel extract was found to be most effective antioxidant in both the test model systems followed by Kufri Jyoti and Kufri Chandramukhi. Chemical analysis revealed the presence of phenolic acids, flavonoids and anthocyanidins as major constituents of these three potent extracts. These potent potato peel extracts were also found to be non-toxic at recommended dosage level (LC50 > 1000 µg/ml). The results provide evidence that peel extract of these three potato varieties, especially Kufri Chipsona-3, may serve as a potential source of natural antioxidants for retarding oxidation of omega-3 fatty acids-enriched food supplement which subsequently may help in developing omega-3 fatty acids fortified foods.

First Page


Last Page




Publication Date