A unique view of SARS-COV-2 through the lens of ORF8 protein


Sk Sarif Hassan
Alaa A.A. Aljabali, Yarmouk University
Pritam Kumar Panda, Uppsala Universitet
Shinjini Ghosh, University of Calcutta
Diksha Attrish, Dr. B.R. Ambedkar Center for Biomedical Research
Pabitra Pal Choudhury, Indian Statistical Institute, Kolkata
Murat Seyran, Universität Wien
Damiano Pizzol, Italian Agency for Development Cooperation - Khartoum
Parise Adadi, University of Otago
Tarek Mohamed Abd El-Aziz, Faculty of Science
Antonio Soares, University of Texas Health Science Center at San Antonio
Ramesh Kandimalla, Indian Institute of Chemical Technology
Kenneth Lundstrom, PanTherapeutics
Amos Lal, Mayo Clinic
Gajendra Kumar Azad, Patna University
Vladimir N. Uversky, Morsani College of Medicine
Samendra P. Sherchan, Tulane University
Wagner Baetas-da-Cruz, Universidade Federal do Rio de Janeiro
Bruce D. Uhal, Michigan State University
Nima Rezaei, Research Center for Immunodeficiencies
Gaurav Chauhan, Tecnologico de Monterrey
Debmalya Barh, Institute of Integrative Omics and Applied Biotechnology (IIOAB)
Elrashdy M. Redwan, King Abdulaziz University
Guy W. Dayhoff, University of South Florida, Tampa
Nicolas G. Bazan, LSUHSC Neuroscience Center
Ángel Serrano-Aroca, Universidad Católica de Valencia San Vicente Mártir
Amr El-Demerdash, Institut de Chimie des Substances Naturelles
Yogendra K. Mishra, University of Southern Denmark, Sønderborg
Giorgio Palu, Università degli Studi di Padova
Kazuo Takayama, Center for iPS Cell Research and Application
Adam M. Brufsky, UPMC Hillman Cancer Center
Murtaza M. Tambuwala, Ulster University

Article Type

Research Article

Publication Title

Computers in Biology and Medicine


Immune evasion is one of the unique characteristics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) attributed to its ORF8 protein. This protein modulates the adaptive host immunity through down-regulation of MHC-1 (Major Histocompatibility Complex) molecules and innate immune responses by surpassing the host's interferon-mediated antiviral response. To understand the host's immune perspective concerning the ORF8 protein, a comprehensive study of the ORF8 protein and mutations possessed by it have been performed. Chemical and structural properties of ORF8 proteins from different hosts, such as human, bat, and pangolin, suggest that the ORF8 of SARS-CoV-2 is much closer to ORF8 of Bat RaTG13-CoV than to that of Pangolin-CoV. Eighty-seven mutations across unique variants of ORF8 in SARS-CoV-2 can be grouped into four classes based on their predicted effects (Hussain et al., 2021) [1]. Based on the geo-locations and timescale of sample collection, a possible flow of mutations was built. Furthermore, conclusive flows of amalgamation of mutations were found upon sequence similarity analyses and consideration of the amino acid conservation phylogenies. Therefore, this study seeks to highlight the uniqueness of the rapidly evolving SARS-CoV-2 through the ORF8.



Publication Date



Open Access, Hybrid Gold, Green

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